Gallery
NextGen 2023
9:00 Registration & Coffee
9:25 David Barford: MRC Laboratory of Molecular Biology
Welcome and Introduction
Session I – Nanocages and Molecular Assemblies
Chair: Chris Johnson, Co-Chair: Stephen McLaughlin
9:30 Jonathan Heddle, University of Durham, UK:
Understanding and building nanomachines from biological molecules
10:00 Lorna Dougan, University of Leeds, UK:
Engineering living hydrogels: from folded proteins to functional protein networks
10:30 Hendrik Dietz Technical University Munich, Germany:
Virus Traps and Other Molecular Machines of the Future
11:00 Coffee Break
Session II – Imaging Across Scales
Chair: Maria Flocco, Co-Chair: Chris Johnson
11:30 Sonja Hess, AstraZeneca, USA:
Integrated Analysis of N-Glycoproteomic and Global Proteomic of Human High-grade Glioblastoma Reveals Distinct Disease Signatures with Potential Clinical Implications
12:00 Radu Aricescu, MRC Laboratory of Molecular Biology, UK:
One needs more structural tools to solve complex biological puzzles
12:30 Clemens Kaminski, University of Cambridge, UK:
Dynamic imaging of organelle dynamics with optical super resolution
1:00 Lunch Break
Session III – Single Molecule Applications
Chair: Chris Batters, Co-Chair: Geoff Holdgate
2:00 Kasia Tych, University of Groningen, Netherlands:
Using single-molecule optical tweezers to study transmembrane proteins – an unfolding story
2:30 Sebastian Deindl, Uppsala University, Sweden:
Illuminating the regulation of gene expression at the single-molecule level
3:00 Coffee Break
Session IV – Nucleic Acid Chemistry and Dynamics
Chair: Geoff Holdgate, Co-Chair: Chris Batters
3:30 Andres Ramos, Institute of Structural and Molecular Biology, UCL, UK:
mRNA modification – from bench to neuron
4:00 Marina Kuimova, Imperial College London, UK:
Mapping microscopic viscosity and temperature using molecular rotors
4:30 Amanda Hargrove, Duke University, USA:
Modulating the conformation and function of disease-relevant RNA with small molecules
5.00 Break
Keynote Lecture
Chair: Maria Flocco, Co-Chair: Stephen McLaughlin
5:10 Matthias Mann, Max-Planck Institute of Biochemistry, Germany:
Technological advances in MS-Based proteomics: Deep Visual and Single Cell Proteomics for precision medicine
6.10 Jan Lowe, Laboratory of Molecular Biology, MRC, UK
Closing remarks
6:15 Drinks Reception
NextGen 2022
9:00 Registration & Coffee
9:25 Welcome and Introduction by Richard Henderson, Laboratory of Molecular Biology, MRC, UK
Session I – RNA Dynamics
Chair: Maria Flocco, Co-Chair: Stephen McLaughlin
9:30 Frédéric Allain, ETH, Zurich, Switzerland:
FUS phase separation, RNA binding and aging as studied by NMR spectroscopy
10:00 David Lilley, University of Dundee, UK:
The extent of RNA catalysis – are there any limits ?
10:30 Katya Petzold, Karolinska Institute, Sweden:
RNA structural changes and their biological impact by NMR
11:00 Coffee Break
Session II – Imaging Across Scales
Chair: Geoff Holdgate, Co-Chair: Chris Johnson
11:30 Andreas Frutiger, Lino Biotech, Zurich, Switzerland:
Label-free interaction analysis in complex samples – focal Molography enables novel applications in bioprocessing, drug discovery and diagnostics
12:00 Tanmay Bharat, Laboratory of Molecular Biology, MRC, UK:
Understanding physiological processes in bacterial cells using cryo-EM
12:30 Jorge Bernardino de la Serna, Imperial College London, UK:
Imaging Cholesterol and its enriched domains spatiotemporally across scales
1:00 Lunch Break
Session III – Single Molecule Applications
Chair: David Rueda, Co-Chair: Geoff Holdgate
2:00 Nynke Dekker, Delft University of Technology, Netherlands:
Dynamic motion during loading and activation of the yeast replisome
2:30 Alice Pyne, University of Shefield, UK:
Uncovering the invisible complexities of the genome using atomic force microscopy
3:00 Ashley Nord, CNRS, Montpellier, France:
Single cell electrical characterization in bacteria
3:30 Coffee Break
Session IV – Computational approaches and Structure Prediction
Chair: Chris Johnson, Co-Chair: Maria Flocco
4:00 John Jumper, DeepMind, London, UK:
AlphaFold and its implications for understanding biology
4:30 Giulia Palermo, University of California Riverside, USA:
Dynamics and Mechanism of CRISPR-Cas9 through the Lens of Computational Methods
5:00 Rhiju Das, Stanford University, USA:
RNA modeling and design
5.30 Break
Keynote Lecture
Chair: Stephen McLaughlin, Co-Chair: David Rueda
5:40 David Baker, University of Washington, USA:
Protein design using deep learning
6.40 Closing Remarks by Jason Chin, Laboratory of Molecular Biology, MRC, UK
6:45 Drinks Reception
NextGen 2021
10:00 Welcome/Introduction by Prof. Jonathan Weber, Dean of the Faculty of Medicine, Imperial College London, UK
Session I – DNA Repair
Chair: Stephen McLaughlin Co-Chair: Chris Johnson
10:10 Simon Boulton, The Francis Crick Institute, UK:
Mechanics of Homologous recombination at a single molecule level
10:40 Lori Passmore, Laboratory of Molecular Biology, MRC, UK:
Insights into DNA crosslink repair through an integrated structural biology approach
11:10 Taiana Maia De Oliveira, AstraZeneca, UK:
Cryo-EM reveals structural insights on DNA Damage Repair
11:40 Coffee Break
Session II – Epigenetics
Chair: Maria Flocco Co-Chair: David Rueda
11:55 Petra Hajkova, London Institute of Medical Sciences, MRC, UK:
The stability and dynamics of DNA modifications in vivo
12:25 Roseana Collepardo, University of Cambridge, UK:
Liquid-like chromatin organization from multiscale modelling
12:55 Lunch Break
Session III – Imaging Across Scales
Chair: Chris Johnson Co-Chair:Maria Flocco
13:30 Hasan Yardimci, The Francis Crick Institute, UK
Visualising chromatin replication one molecule at a time
14:00 Beatrice Dyring-Andersen, University of Copenhagen, Denmark:
Mass spectrometry in clinical dermatology
14:30 Sabrina Leslie, University of British Columbia, Canada:
Single-molecule insights for drug discovery and development: the next level of resolution
15:00 Coffee Break
Session IV –High-throughput Biophysics
Chair: Geoff Holdgate Co-Chair:Chris Johnson
15:15 Philipp Holliger, Laboratory of Molecular Biology, MRC, UK:
High-throughput screening of protein and nucleic acid function
15:45 Chirlmin Joo, TU Delft, Netherlands:
Towards Genome-wide Single-molecule Studies
16:15 William Greenleaf, Stanford University, US:
Understanding the physical genome in health and disease
16:45 Coffee Break
Keynote Lecture
Chair: David Rueda Co-Chair:Stephen McLaughlin
17:00 Patrick Cramer, Max Planck Institute, Germany:
Towards a mechanistic understanding of genome regulation
18:00 Close of Meeting by Jan Lowe, Director of the LMB.
NextGen 2020
All timings are local UK time on October 7th 2020
11.00-11.10: Welcome and opening remarks
Sir Mene Pangalos, EVP Biopharmaceuticals R&D, AstraZeneca.
11.10-12.25: Session I: Imaging Across Scales
Chair: Stephen McLaughlin, LMB. Co-Chair: Christopher Johnson, LMB
11.10-11.35: Andrew Carter. MRC Laboratory of Molecular Biology, UK.
Shulin: a novel factor packaging axonemal outer dynein arms for ciliary targeting
11.35-12.00: Julia Mahamid. EMBL Heidelberg, Germany.
Molecular view into cellular functions by in-cell cryo-electron tomography
12.00-12.25 Richard Goodwin. Drug Discovery AstraZeneca, UK.
Integrated molecular imaging–technologies with the power to offer a new view on the tissue microenvironment and map the efficacy and safety of new therapies
12.25-13.00: Break 35 minutes
13.00-14.15: Session II: Theory and Simulation
Chair: Geoff Holdgate, AstraZeneca. Co-Chair: Christopher Johnson, LMB
13.00-13.25: Martin Depken. TU Delft, Netherlands.
Using mechanistic modelling to improve off-target activity predictions and elucidate the physical basis of SpCas9 fidelity
13.25-13.50: Philip Biggin. Dept. Biochemistry, University of Oxford, UK.
Computational biophysics of trafficking receptors
13.50-14.15: Andres Cisneros, University of North Texas, US
Leveraging Computational Approaches to Uncover and Characterize Cancer-Associated Missense Mutations
14.15-14.30: Break 15 minutes
14.30-15.45: Session III: Biological Phase Separation
Chair: Maria Flocco, AstraZeneca. Co-Chair: David Rueda, Imperial College, London
14.30-14.55: Evan Sprujit. Radboud University, Netherlands.
Understanding biological phase transitions with minimal peptide-based models
14.55-15.20: Priya Banerjee. Dept. Physics. University at Buffalo, USA.
Emergent structure and dynamics of protein-RNA condensates
15.20-15.45: Stephanie Weber. Dept. Biology, McGill University, Canada.
No membrane, no problem: condensing bacterial organelles
15.45-16.00: Break 15 minutes
16.00-17.00 Keynote Lecture
Chair: David Rueda, Imperial College, London. Co-Chair: Maria Flocco, AstraZeneca
Prof. Petra Schwille. Director, Max Plank Institute for Biochemistry, Martinsried, Germany
Is there a minimal set of functions for life?
17.00-17.10 Closing Remarks Jan Löwe, Director, MRC Laboratory of Molecular Biology
NextGen 2019
9:00 Registration & Coffee
9:25 Welcome/Introduction by Mike Snowden, Head of Discovery Sciences, AstraZeneca
Session I – New and Emerging Technologies Chair: Christopher Johnson. MRC-LMB, Cambridge
9:30 Christopher Russo. MRC LMB, Cambridge.
Understanding and approaching some physical limits in CryoEM
10:00 Paul French. Imperial College London.
Multidimensional fluorescence imaging
10:30 Madhavi Krishnan. Chemistry, Oxford.
Bringing electrostatics to light: Electrometry probes a new dimension at the molecular scale
11:00 Coffee Break
Session II – Molecular Conformation and Architecture Chair: Stephen McLaughlin. MRC LMB, Cambridge
11:30 Alireza Mashaghi-Tabari. Leiden University, Netherlands
Dissecting cellular protein folding processes using single-molecule force spectroscopy
12:00 Peter St George-Hyslop, Cambridge Institute for Medical Reasearch
Physiological and Pathological Biological Condensates in Neurons
12:30 Wanda Kukulski. MRC LMB, Cambridge.
Using correlative microscopy and electron cryo-tomography to visualise the molecular architecture of cellular membranes
1:00 Lunch Break
Session III – Single Molecule Applications Chair: David Rueda. Imperial College London
2:00 Stephan Uphoff. Biochemistry, Oxford.
Single-molecule imaging of bacterial DNA repair and mutagenesis
2:30 Anders Gunnarsson. AstraZeneca
Small-molecule drug discovery enabled through dynamic and static single-molecule detection approaches
3:00 Christian Speck. Imperial College London.
Structural and functional analysis of eukaryotic DNA replication
3:30 Coffee Break
Session IV – Molecular Mass Matters. Chair: Maria Flocco, Astra Zeneca
4:00 Justin Benesch. Chemistry, Oxford.
Weighing the evidence for protein structure and dynamics
4:30 Jonathan Wingfield. AstraZeneca
Mass spectrometry at the speed of sound: the application of acoustics for sample loading
Keynote Lecture
5:00 Prof Dame Carol Robinson. Chemistry, Oxford.
From peripheral proteins to membrane motors – mass spectrometry comes of age
5:45 Close of Meeting by Jan Lowe, Director of the MRC LMB
6:00 Drinks Reception
NextGen 2018
9:00 Registration & Coffee
9:25 Welcome/Introduction by Mike Snowden, Head of Discovery Sciences, AstraZeneca
Session I – Nucleic Acid Enzymes and Machines
Chair: David Rueda, Imperial College, London
9:30 Ben Taylor, AstraZeneca, Cambridge:
DNA bubbles induce Cas9 off-target cutting
10:00 Mark Dillingham, School of Biochemistry, Bristol:
The dynamic architecture of ParB-DNA complexes and their role in bacterial chromosome segregation
10:30 Achillefs Kapanidis, Dept of Physics, Oxford:
Discovering transcription mechanisms via single-molecule imaging in vitro and in vivo
11:00 Coffee Break
Session II – Protein Conformation
Chair: Stephen McLaughlin, LMB, Cambridge
11:30 Perdita Barran, School of Chemistry, Manchester:
Using Mass Spectrometry to study IDPs – The perfect tool to examine self-solvation and fuzzy complexes
12:00 Sergi Garcia-Manyes, Kings College, London:
The mechanical stability of proteins regulates their translocation rate into the cell nucleus
12:30 Justin Benesch, Dept of Chemistry, Oxford:
Weighing the evidence for the assembly and dynamics of proteins
1:00 Lunch Break
Session III – New Technologies
Chair: Geoff Holdgate, Astra Zeneca
2:00 Mark Leake, Dept of Physics, York:
Illuminating the black box of DNA-Protein Interactions
2:30 Philipp Kukura, Dept of Chemistry, Oxford:
Weighing single molecules with light
3:00 Steven Lee, Dept of Chemistry, Cambridge: Multidimensional Super-resolution Imaging
3:30 Coffee Break
Session IV – Recognition and Signalling
Chair: Maria Flocco, Astra Zeneca
4:00 Marisa Martin-Fernandez, STFC Central Laser Facility, Harwell:
The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
4:30 Isabel Llorente-Garcia, Dept Physics and Astronomy, UCL, London:
Investigating molecular interactions relevant to virus entry in living cells via optical tweezers force spectroscopy and light-sheet fluorescence microscopy
Keynote Lecture
5:00 Richard Henderson, LMB, Cambridge:
Single Particle cryoEM technology
5:45 Close of Meeting by Jan Lowe, Director of the MRC-LMB
6:00 Drinks Reception
NextGen 2017
9.00 – 9.15 Arrive
9.15 – 9.30 Welcome / Introduction by Mike Snowden, Head of Discovery Sciences, AstraZeneca
Session I, Chairperson: Geoff Holdgate
9.30 – Bart Hoogenboom, London Centre for Nanotechnology, University College London: Probing individual biomolecules and biomolecular assemblies with better than super-resolution
10.00 – Stefan Geschwindner, AstraZeneca, Gothenburg, Sweden: Seeing drugs in action: From molecular ensembles to single molecules with TIRF microscopy
10.30 – Robert Henderson, Dept. Pharmacology, University of Cambridge: Molecules and movement: Studies using fast-scan atomic force microscopy
11.00 – 11.30 Coffee
Session II, Chairperson: Stephen McLaughlin
11.30 – Charlotte Dodson, National Heart & Lung Institute, Imperial College London: Dynamic equilibrium of Aurora-A kinase activation loop revealed by single molecule spectroscopy
12.00 – Luca Pellegrini, Dept. Biochemistry, University of Cambridge: Biophysical studies of the meiotic protein SYCP3
12.30 – David Rueda, Dept. Medicine, Imperial College London: Watching protein-nucleic acid interactions with single molecule resolution
1.00 – 2.00 – Lunch
Session III, Chairperson: Maria M. Flocco
2.00 – Roger Williams LMB, Cambridge: Using hydrogen/deuterium exchange mass spectrometry to understand regulation of cellular signalling
2.30 – Justin Molloy, Crick Institute, London: Use of optically-based single molecule techniques to study molecular machines
3.00 – David Klenerman, Dept. Chemistry, University of Cambridge: Single molecule studies of neurodegeneration
3.30 – 4.00 Coffee
Session IV, Chairperson: Chris Johnson
4.00 – Bruce Carrington, UCB Pharma, Slough: Structural and biophysical techniques to investigate protein conformations in PPI drug discovery
4.30 – Sonia Antoranz Contera, Dept. of Physics, University of Oxford: AFM and beyond: Forces and mechanics to image, quantify and steer biological function from single molecules to cells and living tissues
5.00 – Emmanuel Derivery, LMB, Cambridge: Polarized endosome dynamics by spindle asymmetry during asymmetric cell division
5.30 – Close of Meeting by Sir Hugh Pelham, Director of the MRC-LMB
Drinks Reception